Changes in Soil Nutrients and Acidobacteria Community Structure in Cunninghamia lanceolata Plantations

Objective:This paper investigated the variation of soil nutrients and oligotrophic bacterium, Acidobacterium communities during continuous planting of Cunninghamia lanceolata plantation in Kaihua, Zhejiang Province, to reveal the molecular ecological mechanism of Acidobacterium causing soil fertility changes in different types of C. lanceolata plantations. This study aimed to provide scientific basis for the adjustment of C. lanceolata stand structure, the scientific management of soil resources as well as the construction of healthy soil ecosystem in this area.Method: The soil samples at different depths (0-20 cm, 20-40 cm) were collected from three different plantations: Pinus massoniana plantation(served as control); the first rotation of C. lanceolata plantation established after the clear cutting of P. massoniana, and the natural regeneration second rotation of C. lanceolata plantation, in Kaihua Forestry Farm in Zhejiang. Nutrient contents, physical and chemical properties of soil were analyzed. Then, the bacterial 16S rDNA in soil was analyzed using high-throughput sequencing.Result: 1)The replacement of P. massoniana plantation with C. lanceolata plantation led to a significant decrease in pH value, alkali-hydrolyzable nitrogen, available phosphorus and available kalium content(Pthe first-rotation C. lanceolata plantation>the second-rotation C. lanceolata plantation. 2) On the phyla level, Acidobacteria were dominant in soil bacteria of the three forest types, accounting for 32.68%-49.17%. The Acidobacteria proportion in 0-20 cm soil layer of the second-rotation C. lanceolata plantation was significantly higher than that of P. massoniana plantation (P © 2019, Editorial Department of Scientia Silvae Sinicae. All right reserved.