Fabrication of LSPR Sensors Based on Branched Gold Nanoparticles for Immunoassays

被引：0
|
作者：
Su R. [1 ,2 ,3 ]
Tang Y. [1 ,2 ]
Yin H. [1 ,2 ]
Huang R. [1 ,2 ]
Qi W. [1 ,2 ,3 ]
He Z. [1 ,2 ]
机构：
[1] School of Chemical Engineering and Technology, Tianjin University, Tianjin
[2] State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin
[3] Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin
来源：
Su, Rongxin (surx@tju.edu.cn) | 1600年 / Tianjin University卷 / 53期
基金：
中国国家自然科学基金;
关键词：
Branched structure; IgG; Polydopamine(PDA); Surface plasmon resonance;
D O I：
10.11784/tdxbz201904014
中图分类号：
学科分类号：
摘要：
Branched gold nanostructures have unique sharp tips and "hot spot", which can strongly enhance their surrounding electric fields. As a result, branched nanoparticles have strong local surface plasmon resonance (LSPR) signals. Herein, gold nanomaterials with branched nanostructures were in situ synthesized on an optical fiber, and optical fiber LSPR sensors were fabricated and applied for immunoassays. An adhesive layer of polydopamine (PDA) was first prepared on the preprocessed optical fiber and then applied to bind gold seeds. Based on in situ reduction and silver induction, gold nanomaterials with branched nanostructures were fabricated on an optical fiber. The temperatures and time of dopamine polymerization, as well as the gold plating time, were optimized. The optimized values are set as 10℃, 15min, and 5min. The LSPR sensor fabricated under optimal condition showed a high sensitivity of 4091nm/RIU with a refractive index ranging from 1.333 to 1.381. Moreover, high stability of the fabricated sensor was demonstrated by comparing the spectrum of the original sensor with the spectra of the sensor after solvent rinsing, scotch tape tearing, and immersion in piranha solution. In addition, 0.01% gold chloride was applied as a plating solution, whose concentration was only one-tenth of that of the traditional methods. As a result, the presented method is more economical. Moreover, HIgG was immobilized on the gold film to detect RAHIgG. The fabricated biosensor reached a detection sensitivity of 0.086(nm/μg)•mL during the RAHIgG concentration of 0-75μg/mL. © 2020, Editorial Board of Journal of Tianjin University(Science and Technology). All right reserved.
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页码：441 / 449
页数：8
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