Effect of pH on the Interaction of Caffeic Acid β-Phenethyl Ester with Human Serum Albumin as Studied by Fluorescence Spectroscopy

被引：0
|
作者：
Fan J. [1 ]
Xing L. [1 ]
Li M. [1 ]
Ma A. [1 ]
Lü C. [1 ]
Li J. [1 ]
机构：
[1] Food Safety Key Laboratory of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University,
来源：
Li, Jianrong (lijr6491@163.com) | 1600年 / Chinese Chamber of Commerce卷 / 42期
关键词：
Caffeic acid β-phenethyl ester; Fluorescence quenching; Human serum albumin; PH;
D O I：
10.7506/spkx1002-6630-20201109-076
中图分类号：
学科分类号：
摘要：
In order to explore the effect of pH on the interaction of caffeic acid β-phenethyl ester (CAPE) with human serum albumin (HSA), the quenching effect of CAPE on the fluorescence of HSA under different pH (3.0, 5.0 and 7.4) conditions was investigated using fluorescence emission spectroscopy (FES) and synchronous fluorescence spectroscopy (SFS), and the quenching constants, binding constants and thermodynamic parameters were calculated. Meanwhile, the fluorescence quenching mechanism was analyzed, and the binding site was determined by site marker experiment. The fluorescence emission spectra showed that CAPE could quench the intrinsic fluorescence of HSA under all pH conditions tested. The quenching rate was the highest and the binding constant was the largest at physiological pH 7.4.The thermodynamic results suggested that the binding of CAPE to HSA was mainly driven by van der Waals force and hydrogen bonds.SFS indicated that binding to CAPE and the change of pH jointly induced the conformational changes of HSA. The results of site marker experiment showed that the binding site of CAPE to HSA was located near Sudlow's site I. Altogether, the results revealed that the combination of CAPE with HSA could be promoted by the increase of pH, which can provide a theoretical basis for activity homeostasis maintenance and targeted delivery of CAPE. © 2021, China Food Publishing Company. All right reserved.
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页码：31 / 37
页数：6
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