Identification of a Heat-Resistant Strain of Bacillus cereus and Evaluation of Its Efficacy in Alleviating Inflammatory Bowel Disease

被引：0
|
作者：
Xu Y. [1 ]
Sheng K. [1 ]
Wang Y. [1 ]
机构：
[1] Key Laboratory of Human Microecology and Precision Medicine of Anhui Province, College of Life Sciences, Anhui University, Hefei
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 02期
关键词：
Bacillus cereus; Conserved protein-coding genes; Growth characteristics; Inflammatory bowel disease; Virulence genes;
D O I：
10.7506/spkx1002-6630-20220316-176
中图分类号：
学科分类号：
摘要：
An unknown laboratory strain was identified and named as Bacillus cereus HMPM18123 based on the 16S rRNA gene and conserved protein-coding gene (gyrA and gyrB) sequences combined with virulence genes (nheA, nheB, nheC, hblA, hblC, hblD, becT, cytK and entFM) and biochemical properties. The tolerance of the strain to different temperatures, pH, simulated gastric and intestinal fluid was evaluated as well as its efficacy in alleviating colitis induced by dextran sulfate sodium (DSS) in mice. The results of growth characteristics showed that the strain had good tolerance to high temperature and simulated gastric and intestinal fluid. In addition, the strain improved DSS-induced colitis symptoms in mice such as body mass loss, colon shortening, increased disease activity index (DAI) score, tissue lesions, and up-regulated expression of inflammatory factors. © 2023, China Food Publishing Company. All right reserved.
引用
收藏
页码：173 / 180
页数：7
相关论文
共 32 条
  • [1] ARNESEN L P S, FAGERLUND A, GRANUM P E, Et al., From soil to gut: Bacillus cereus and its food poisoning toxins, FEMS Microbiology Reviews, 32, 4, pp. 579-606, (2008)
  • [2] DIERICK K, VAN COILLIE E, SWIECICKA I, Et al., Fatal family outbreak of Bacillus cereus-associated food poisoning, Journal of Clinical Microbiology, 43, 8, pp. 4277-4279, (2005)
  • [3] HONG H A, DUC L H, CUTTING S M., The use of bacterial spore formers as probiotics, FEMS Microbiology Reviews, 29, 4, pp. 813-835, (2005)
  • [4] DE SARRAU B, CLAVEL T, CLERTE C, Et al., Influence of anaerobiosis and low temperature on Bacillus cereus growth, metabolism, and membrane properties, Applied and Environmental Microbiology, 78, 6, pp. 1715-1723, (2012)
  • [5] KAMINSKA P S, YERNAZAROVA A, DREWNOWSKA J M, Et al., The worldwide distribution of genetically and phylogenetically diverse Bacillus cereus isolates harbouring Bacillus anthracis-like plasmids, Environmental Microbiology Reports, 7, 5, pp. 738-745, (2015)
  • [6] YAMAMOTO S, BOUVET P J M, HARAYAMA S., Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization, International Journal of Systematic & Evolutionary Microbiology, 49, pp. 87-95, (1999)
  • [7] SOLER L, YANEZ M A, CHACON M R, Et al., Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes, International Journal of Systematic and Evolutionary Microbiology, 54, 5, pp. 1511-1519, (2004)
  • [8] CHUN J, BAE K S., Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences, Antonie van Leeuwenhoek, 78, 2, pp. 123-127, (2000)
  • [9] DAUGA C., Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies, International Journal of Systematic and Evolutionary Microbiology, 52, 2, pp. 531-547, (2002)
  • [10] 31, (2019)
1234