Separation and Identification of Yeast from Traditional Shrimp Paste and Their Carbon Utilization Characteristics

被引：0

作者：

Xu W. ^{[1
]}

Wu R. ^{[1
]}

Li C. ^{[1
]}

Lü J. ^{[1
]}

Ji C. ^{[1
]}

Liang H. ^{[1
]}

Sun Z. ^{[1
]}

Lin X. ^{[1
]}

机构：

[1] National Engineering Research Center of Seafood, School of Food, Dalian Polytechnic University, Dalian

来源：

Journal of Chinese Institute of Food Science and Technology | 2021年 / 21卷 / 04期

关键词：

26S rDNA; Carbon utilization; Separation and identification; Shrimp paste; Yeast;

D O I：

10.16429/j.1009-7848.2021.04.036

中图分类号：

学科分类号：

摘要：

In this study, yeasts were purified from traditional shrimp paste by gradient dilution method and their carbon source utilization ability were studied. After extraction of genomic DNA and PCR amplification, 26S rDNA sequence analysis for these yeasts were carried out. Four kinds of yeasts were identified, which were Rhodotorula mucilaginosa, Candida parapsilosis, Trichosporon domesticum and Debaryomyces hansenii, respectively. Carbon source utilization was evaluated with carbon source assimilation analysis. Results showed that the utilization ability of carbon source from strong to weak was as follow: glucose = sucrose> glucosamine> sorbitol> glycerol> mannitol> citric acid> lactose> chitosan. Glucose and sucrose were the optimal carbon sources, which can support the growth of all yeasts. Glucose and sucrose can support all yeast growth, and the remaining carbon sources can support yeast growth to varying degrees. This study provides the microbial resource from shrimp paste. © 2021, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：303 / 309

页数：6

共 23 条

[1] 9
[2] 1
[3] 2
[4] 8
[5] 8
[6] 3, pp. 40-43
[7] 7, pp. 92-97
[8] 7
[9] AKSU Z, EREN A T B., Carotenoids production by the yeast Rhodotorula mucilaginosa: Use of agricultural wastes as a carbon source, Process Biochemistry, 40, 9, pp. 2985-2991, (2005)
[10] BAO R, WU X, LIU S, Et al., Efficient conversion of fructose-based biomass into lipids with Trichosporon fermentans under phosphate-limited conditions, Applied Biochemistry and Biotechnology, 184, 1, pp. 113-123, (2017)

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