Protective Effect of Curcumin/Cyclodextrin Polymer Inclusion Complex on LO2 Cells Damaged by Ethanol

被引：0
|
作者：
Fan T. [1 ,2 ]
Chen J. [1 ,2 ]
Gao J. [1 ,2 ]
Zhong S. [1 ,2 ]
Qin X. [1 ,2 ]
机构：
[1] College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laborat
[2] Dalian Polytechnic University, Collaborative Innovation Center of Seafood Deep Processing, Dalian
来源：
Science and Technology of Food Industry | 2021年 / 42卷 / 18期
关键词：
curcumin/cyclodextrin polymer inclusion complex; ethanol-induced injury; LO2; cells; protective effect; reactive oxygen species;
D O I：
10.13386/j.issn1002-0306.2020120083
中图分类号：
学科分类号：
摘要：
This study investigated the protective effect of curcumin/cyclodextrin polymer inclusion complex(CUR/CDP) on LO2 cells damaged by ethanol. The MTT method was used to establish a model of LO2 cells damaged by ethanol. Glutamate alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH), superoxide dismutase(SOD), glutathione peroxidase(GSH-PX), malondialdehyde(MDA) and reactive oxygen species(ROS) kits were used to investigate the protective effect of CUR/CDP on LO2 cells damaged by ethanol. The results showed that after CUR/CDP (80 μg/mL) treatment, the cell activity of LO2 cells increased from (54.75%±8.97%) (model group) to 85.27%±2.64%, and the activities of ALT, AST, and LDH in the LO2 cell culture medium decreased from (26.47±0.90), (41.02±4.41), (63.77±4.95) U/L (model group) to (16.17±0.42), (22.62±0.79), (32.25±1.69) U/L (P<0.01), respectively; the activities of GSH-PX and SOD in LO2 cells increased from (21.82±1.34), (8.45±1.11) U/mg prot (model group) to (36.70±0.56), (16.47±1.27) U/mg prot; the content of MDA and ROS in LO2 cells decreased from (1.19±0.15) nmol/mg prot, (198.02%±11.76%) (model group) to (0.72±0.05) nmol/mg prot, (110.87%±10.22%) (P<0.01), respectively. In conclusion, CUR/CDP could improve the damage of LO2 cells induced by ethanol by increasing the activity of antioxidant enzymes and reducing the content of ROS. © 2021 Editorial Department of Science and Technology of Food Science. All rights reserved.
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页码：366 / 371
页数：5
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