Effect of Berberine on the mRNA Expression of Nitric Oxide Synthase(NOS) in Rat Corpus Cavernosum

被引：0
|
作者：
谭艳
明章银
汤强
蒋兆键
胡本容
向继洲
机构：
[1] China 2Department of Pharmacology
[2] Shiyan 442000
[3] Wuhan 430030
[4] School of Basic Medical Sciences
[5] Huazhong University of Science and Technology
[6] Yunyang Medical College
[7] China
[8] Department of Pharmacology
[9] Tongji Medical College
来源：
Current Medical Science | 2005年 / 02期
基金：
中国国家自然科学基金;
关键词：
berberine; nitric oxide synthase; nitric oxide; corpus cavernosum; erectile dysfunction;
D O I：
暂无
中图分类号：
R285 [中药药理学];
学科分类号：
1008 ;
摘要：
Summary: In order to further investigate the mechanisms of action of berberine (Ber), we assessed the effects of Ber on the mRNA expression of nitric oxide synthases (NOS) in rat corpus cavernosum. After incubation with Ber for 1 or 3 h respectively, the levels of NOS mRNA were examined by reverse transcription polymerase chain reaction (RT-PCR). Our results showed that there were iNOS and eNOS mRNA expressions in rat corpus cavernosum. Ber enhanced eNOS mRNA expression in rat penis, but exhibited no effect on the expression of iNOS mRNA (P>0.05). The present study indicated that the relaxation of Ber involved the NO-cGMP signal thansduction pathway. The enhancing effect of Ber on eNOS mRNA expression might associated with its relaxation of corpus cavernosum.
引用
收藏
页码：127 / 130
页数：4
相关论文
共 50 条
  • [1] Effect of berberine on the mRNA expression of nitric oxide synthase (NOS) in rat corpus cavernosum
    Tan Yan
    Ming Zhangyin
    Tang Qiang
    Jiang Zhaojian
    Hu Benrong
    Xiang Jizhou
    Current Medical Science, 2005, 25 (2) : 127 - 130
  • [2] Effects of androgens on the expression of nitric oxide synthase mRNAs in rat corpus cavernosum
    Park, KH
    Kim, SW
    Kim, KD
    Paick, JS
    BJU INTERNATIONAL, 1999, 83 (03) : 327 - 333
  • [3] Relationship between gene expression of nitric oxide synthase and androgens in rat corpus cavernosum
    沈周俊
    陈昭典
    陆颖理
    陈峰
    陈智
    ChineseMedicalJournal, 2000, (12)
  • [4] Relationship between gene expression of nitric oxide synthase and androgens in rat corpus cavernosum
    Shen, ZJ
    Chen, ZD
    Lu, YL
    Chen, F
    Chen, Z
    CHINESE MEDICAL JOURNAL, 2000, 113 (12) : 1092 - 1095
  • [5] Relationship between gene expression of nitric oxide synthase and androgens in rat corpus cavernosum
    沈周俊
    陈昭典
    陆颖理
    陈峰
    陈智
    中华医学杂志(英文版), 2000, (12) : 36 - 39
  • [6] Effect of diabetes and insulin treatment on nitric oxide synthase content in rat corpus cavernosum
    Xu, ZS
    Fu, QG
    Zhao, ST
    Liu, HN
    ASIAN JOURNAL OF ANDROLOGY, 2001, 3 (02) : 139 - 142
  • [7] Hydrogen sulfide promotes nitric oxide production in corpus cavernosum by enhancing expression of endothelial nitric oxide synthase
    Meng, J.
    Adaikan, P. Ganesan
    Srilatha, B.
    INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH, 2013, 25 (03) : 86 - 90
  • [8] Hydrogen sulfide promotes nitric oxide production in corpus cavernosum by enhancing expression of endothelial nitric oxide synthase
    J Meng
    P Ganesan Adaikan
    B Srilatha
    International Journal of Impotence Research, 2013, 25 : 86 - 90
  • [9] The Effects of Beta-blockers on Endothelial Nitric Oxide Synthase Immunoreactivity in the Rat Corpus Cavernosum
    Dogru, Mehmet Tolga
    Aydos, Tolga Resat
    Aktuna, Zuhal
    Korkusuz, Petek
    Zeybek, Dilara
    Gorgu, Nart
    Korkut, Oguzhan
    Basar, Mehmet Murad
    UROLOGY, 2010, 75 (03) : 589 - 597
  • [10] Expression of nitric oxide synthase (NOS) in human and rat gastric tissue
    Fischer, H
    Becker, JC
    Boknik, P
    Huber, V
    Konturek, JW
    Luss, H
    Neumann, J
    Schmitz, W
    Domschke, W
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1997, 355 (04) : 496 - 496
12345