An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements

This study compared the biological changes of lipopolysaccharide(LPS)-treated dental pulp(DP)cells directly cultured on mineral trioxide aggregate(MTA)and calcium silicate(CS)cements.DP cells were treated with LPS for 24 h.Then,the LPS-treated DP cells were cultured on MTA or CS cements.Cell viability,cell death mechanism and interleukin(IL)-1b expressions were analysed.A one-way analysis of variance was used to evaluate the significance of the differences between the means.A significantly higher IL-1b expression(2.9-fold)was found for LPS-treated cells(P,0.05)compared with DP cells without LPS treatment at 24 h.Absorbance values of LPS-treated cells cultured on CS cement were higher than a tissue culture plate.A significant difference(P,0.05)in cell viability was observed between cells on CS and MTA cements 24 h after seeding.At 48 h,a high concentration of Si(5 mM)was released from MTA,which induced LPS-treated DP cell apoptosis.The present study demonstrates that CS cement is biocompatible with cultured LPS-treated DP cells.MTA stimulates inflammation in LPS-treated DP cells,which leads to greater IL-1b expression and apoptosis.