STUDY ON THE FUNCTIONAL SITES OF Mg2+ -INDUCED Chl α FLUORESCENCE AND SURFACE CHARGE CHANGES OF THYLAKOID MEMBRANES

Thylakoid polypeptide components, Mg-induced Chl a fluorescence at 77K and surface chargechanges were measured to investigate the functional sites of Mgin the regulation of energy distri-bution between two photosystems in completely and incompletely developed barley chloroplasts. It wasfound that in contrast to the completely developed chloroplasts, the incompletely developed chloroplastslacked Chl b and did not contain the 23KDa and 25KDa polypeptide components of LHC-PSII. In themeantime, these membranes did nor present Mg-induced Cbl a fluorescence and surface charge changesof thylakoids. These results provided strong evidence that the 23KDa and 25KDa polypeptides ofLHC-PSII are the specific acting sites of the cation that induced these two phenomena. It is suggested in this paper that Mg-induced change of excitation energy distribution betweentwo photosystems is produced by the mechanism due to electrostatic neutralization of LHC-PSII bythe cation to cause a structural or con