THE T/T COMMON REGION OF SIMIAN VIRUS-40 LARGE T-ANTIGEN CONTAINS A DISTINCT TRANSFORMATION-GOVERNING SEQUENCE

被引:49
|
作者
MARSILIO, E
CHENG, SH
SCHAFFHAUSEN, B
PAUCHA, E
LIVINGSTON, DM
机构
[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115
[3] HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115
[4] GENZYME CORP,CELLULAR REGULAT LAB,FRAMINGHAM,MA 01701
[5] TUFTS UNIV,DEPT BIOCHEM,BOSTON,MA 02111
关键词
D O I
10.1128/JVI.65.10.5647-5652.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Simian virus 40 large T antigen (T) can transform cultured cells, but the mechanisms by which it functions are not entirely understood. Several lines of evidence have suggested that the amino-terminal approximately 130 residues of T may be sufficient to confer the transforming capability. Oligonucleotide-directed mutagenesis was used to generate a series of deletion and substitution mutants within the amino-terminal 82 residues of T, the segment which is shared with simian virus 40 small t antigen (t). Results of stability and transformation assays of these mutants strongly suggest that the 1-to-82 region of T contains sequences which govern T transforming activity and affect in vivo stability. Instability and a defect in transforming activity could be separated from one another genetically. Thus, the 1-to-82 region appears to contain a specific region that contributes to the transforming function of the protein. This segment operates by means other than the simple binding of pRb and/or p107.
引用
收藏
页码:5647 / 5652
页数:6
相关论文
共 50 条