The aim of the present study was to quantitatively investigate the role of information processing in conditioning using stimuli with varying amounts of information content. A letter reproduction task varying in complexity served as the unconditioned stimulus. In the first experiment, we tested the indicator function of electrodermal and cardiovascular variables. The amount of information processing resources required emerged most clearly in electrodermal reactions (EDRs), showing an increase with increasing stimulus information content. A second experiment was performed using the same design to confirm the role of processing resources allocation during conditioning by means of an independent indicator. A reaction time task was introduced as a measure of information processing resources utilized. The results paralleled the dependence of EDRs on stimulus information content, as was found in the first experiment.