LOCALIZATION OF A LOW MR GTP-BINDING PROTEIN, RAP1 PROTEIN, IN PLASMA-MEMBRANES AND SECRETORY GRANULE MEMBRANES OF RAT PAROTID-GLAND

被引：7
|
作者：
KAMEYAMA, Y [1 ]
NAGATA, K [1 ]
MIZUNOKAMIYA, M [1 ]
YOKOTA, Y [1 ]
FUJITA, A [1 ]
NOZAWA, Y [1 ]
机构：
[1] GIFU UNIV,SCH MED,DEPT BIOCHEM,GIFU 500,JAPAN
来源：
LIFE SCIENCES | 1994年 / 55卷 / 03期
关键词：
LOW MR GTP-BINDING PROTEIN; SECRETORY GRANULE MEMBRANES; PLASMA MEMBRANE; PAROTID GLAND;
D O I：
10.1016/0024-3205(94)00882-5
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Subcellular fractions were prepared from rat parotid gland by sequential centrifugation, Percoll gradient centrifugation and divalent-cation precipitation, and the localization of a low Mr GTP-binding protein, rap1 protein (rap1p) was analyzed by immunoblotting using a specific antibody. rap1p was found to be located in apical and basolateral plasma membranes, and secretory granule membranes in rat parotid gland. On the other hand, the beta gamma subunits of heterotrimeric Gt protein was localized in plasma membranes but not in granule membranes.
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页码：213 / 219
页数：7
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