MEASUREMENT OF MALONDIALDEHYDE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引：273
|
作者：
YOUNG, IS
TRIMBLE, ER
机构：
[1] Department of Clinical Biochemistry, Queen's University of Belfast, Belfast BT12 6BA, Grosvenor Road
来源：
ANNALS OF CLINICAL BIOCHEMISTRY | 1991年 / 28卷
关键词：
LIPID PEROXIDATION; THIOBARBITURIC ACID; FREE RADICALS;
D O I：
10.1177/000456329102800514
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
The thiobarbituric acid test for malondialdehyde is widely used as an indicator of lipid peroxidation and free radical activity. We describe a simple and sensitive high performance liquid chromatographic (HPLC) method for measuring the malondialdehyde-thiobarbituric acid adduct using fluorimetric detection. Extraction into organic solvent or other complex sample preparation is not required and the assay is sensitive to < 0.05-mu-mol/L. This method could easily be applied to any setting where a measure of lipid peroxidation is required.
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页码：504 / 508
页数：5
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