ACTION OF PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE ON INVITRO PHEROMONE GLANDS OF HELIOTHIS-ARMIGERA FEMALES

被引：78

作者：

RAFAELI, A ^{[1
]}

SOROKER, V ^{[1
]}

KAMENSKY, B ^{[1
]}

RAINA, AK ^{[1
]}

机构：

[1] USDA ARS,BELTSVILLE AGR RES CTR,INSECT CHEM ECOL LAB,BELTSVILLE,MD 20705

来源：

JOURNAL OF INSECT PHYSIOLOGY | 1990年 / 36卷 / 09期

关键词：

cyclic-AMP; Heliothis armigera; Pheromone Biosynthesis Activating Neuropeptide (PBAN); pheromone glands;

D O I：

10.1016/0022-1910(90)90068-Q

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Pheromone glands of Heliothis armigera were stimulated in vitro to incorporate 14C from the radioactive precursor sodium acetate, in the presence of synthetic pheromone biosynthesis activating neuropeptide (PBAN). When hexane extracts of the radioactive products were analysed by TLC and HPLC the radioactivity corresponded in retention time to the main pheromone component (Z)-11-hexadecenal. Maximal stimulation, as depicted by TLC analysis, was observed after 4 h of incubation. The PBAN response was shown to be dose dependent, maximal levels, as analysed by TLC, were obtained at a concentration of 5 pmol/gland. This response was shown to be mediated by cAMP. © 1990.

引用

页码：641 / 646

页数：6

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