A NEW ULTRASENSITIVE BIOLUMINOGENIC ENZYME SUBSTRATE FOR BETA-GALACTOSIDASE

被引：36
|
作者：
GEIGER, R
SCHNEIDER, E
WALLENFELS, K
MISKA, W
机构：
[1] UNIV FREIBURG,INST ORGAN CHEM & BIOCHEM,W-7800 FREIBURG,GERMANY
[2] UNIV GIESSEN,DERMATOL & ANDROL ABT,W-6300 GIESSEN,GERMANY
来源：
BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1992年 / 373卷 / 12期
关键词：
D-LUCIFERIN; D-LUCIFERIN DERIVATIVES; D-LUCIFERIN-O-BETA-GALACTOSIDE; LUCIFERASE; BIOLUMINESCENCE; FIREFLY; PHOTINUS-PYRALIS AND BETA-GALACTOSIDASE;
D O I：
10.1515/bchm3.1992.373.2.1187
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A derivative of D-luciferin, D-luciferin-O-beta-galactoside, was synthesized and used as highly sensitive substrate for beta-galactosidase. The substrate was physicochemically characterized. Enzymatic cleavage of the new compound by beta-galactosidase was demonstrated and kinetic constants K(m), V(max), k(cat) and k(cat)/K(m) K(m) have been determined. The compound has been proved to be a highly sensitive substrate for beta-galactosidase, permitting a limit of detection of 3.7 x 10(-19) mol of enzyme per assay.
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页码：1187 / 1191
页数：5
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