BEHAVIOR OF POLYHYDROXYETHYL METHACRYLATE SORBENT WITH DEXTRAN-FILLED MACROPORES IN DYE-AFFINITY CHROMATOGRAPHY OF PROTEINS

A series of composite sorbents based on the rigid macroporous polyhydroxyethyl methacrylate (HEMA)-based support filled with soft dextran gel was prepared. The affinity dyes Cibacron Blue and Remazol Brilliant Blue were then immobilized on both the original HEMA and the composite (HEMA-D) sorbents. The properties of HEMA and HEMA-D sorbents, such as hydrophobic interactions with proteins and recovery of chromatographed enzymes (lactate dehydrogenase and malate dehydrogenase), in both original and dyed forms were studied. Important advantages of using HEMA-D supports in dye-affinity chromatography as compared with only HEMA supports were found.