PRELIMINARY CRYSTALLOGRAPHIC STUDY OF PHOSPHOLIPASE-A2 FROM THE VENOM OF TRIMERESURUS-FLAVOVIRIDIS (HABU SNAKE)

被引：3
|
作者：
SHINODA, Y
SHOUJI, A
YAMANE, T
SUZUKI, A
ASHIDA, T
KIHARA, H
OHNO, M
机构：
[1] NAGOYA UNIV,FAC ENGN,DEPT APPL CHEM,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN
[2] KYUSHU UNIV,FAC SCI,DEPT CHEM,HIGASHI KU,FUKUOKA 812,JAPAN
[3] KAGOSHIMA UNIV,FAC MED,DEPT PHYSIOL,KAGOSHIMA 890,JAPAN
来源：
JOURNAL OF BIOCHEMISTRY | 1990年 / 107卷 / 01期
关键词：
D O I：
10.1093/oxfordjournals.jbchem.a123017
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Crystallization and a preliminary crystallographic study of Trimeresurus flavoviridis (habu snake) phospholipase A2 (PLA2) were carried out. Although crystals were obtained from various solutions, crystals suitable for X-ray analysis could be obtained from polyethylene glycol solutions only when a repeated seeding technique was applied starting from twinned crystals. The crystal is monoclinic with space group P21, with a = 44.1, b = 55.7, c = 48.8A, and β = 92.4. An asymmetric unit contains a dimer consisting of two identical subunits made of 122 amino acids. The crystal reflects X-rays beyond 2.5 A. A Pt derivative gave a good isomorphous crystal. © 1990 COPYRIGHT, 1990 BY THE JOURNAL OF BIOCHEMISTRY.
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页码：84 / 86
页数：3
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