CAMP-DEPENDENT PROTEIN-KINASE - CRYSTALLOGRAPHIC INSIGHTS INTO SUBSTRATE RECOGNITION AND PHOSPHOTRANSFER

被引:0
|
作者
MADHUSUDAN
TRAFNY, EA
XUONG, NH
ADAMS, JA
TENEYCK, LF
TAYLOR, SS
SOWADSKI, JM
机构
[1] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093
[2] UNIV CALIF SAN DIEGO,DEPT CHEM,LA JOLLA,CA 92093
[3] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093
[4] UNIV CALIF SAN DIEGO,DEPT PHYS,LA JOLLA,CA 92093
[5] SAN DIEGO SUPERCOMP CTR,SAN DIEGO,CA 92186
关键词
CRYSTAL STRUCTURE; GLYCINE-RICH LOOP; PHOSPHOTRANSFER; PROTEIN KINASES; SUBSTRATE RECOGNITION;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The crystal structure of ternary and binary substrate complexes of the catalytic subunit of cAMP-dependent protein kinase has been refined at 2.2 and 2.25 Angstrom resolution, respectively. The ternary complex contains ADP and a 20-residue substrate peptide, whereas the binary complex contains the phosphorylated substrate peptide. These 2 structures were refined to crystallographic R-factors of 17.5 and 18.1%, respectively. In the ternary complex, the hydroxyl oxygen OG of the serine at the P-site is 2.7 Angstrom from the OD1 atom of Asp 166. This is the first crystallographic evidence showing the direct interaction of this invariant carboxylate with a peptide substrate, and supports the predicted role of Asp 166 as a catalytic base and as an agent to position the serine -OH for nucleophilic attack. A comparison of the substrate and inhibitor ternary complexes places the hydroxyl oxygen of the serine 2.7 Angstrom from the gamma-phosphate of ATP and supports a direct in-line mechanism for phosphotransfer. In the binary complex, the phosphate on the Ser interacts directly with the epsilon N of Lys 168, another conserved residue. In the ternary complex containing ATP and the inhibitor peptide, Lys 168 interacts electrostatically with the gamma-phosphate of ATP (Zheng J, Knighton DR, Ten Eyck LF, Karlsson R, Xuong NH, Taylor SS, Sowadski JM, 1993, Biochemistry 32:2154-2161). Thus, Lys 168 remains closely associated with the phosphate in both complexes. A comparison of this binary complex structure with the recently solved structure of the ternary complex containing ATP and inhibitor peptide also reveals that the phosphate atom traverses a distance of about 1.5 Angstrom following nucleophilic attack by serine and transfer to the peptide. No major conformational changes of active site residues are seen when the substrate and product complexes are compared, although the binary complex with the phosphopeptide reveals localized changes in conformation in the region corresponding to the glycine-rich loop. The high B-factors for this loop support the conclusion that this structural motif is a highly mobile segment of the protein.
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页码:176 / 187
页数:12
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