ENUMERATION OF VIBRIO-VULNIFICUS ON MEMBRANE FILTERS WITH A FLUORESCENTLY LABELED OLIGONUCLEOTIDE PROBE SPECIFIC FOR KINGDOM-LEVEL 16S RIBOSOMAL-RNA SEQUENCES

被引：16

作者：

HEIDELBERG, JF

ONEILL, KR

JACOBS, D

COLWELL, RR

机构：

[1] UNIV MARYLAND,MARINE ESTUARINE ENVIRONM SCI PROGRAM,COLL PK,MD 20742

[2] UNIV MARYLAND,MARYLAND SEA GRANT COLL,COLL PK,MD 20742

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 10期

关键词：

D O I：

10.1128/AEM.59.10.3474-3476.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Vibrio vulnificus was enumerated on membrane filters after hybridization with a fluorescent oligonucleotide eubacterial probe. Cells were hybridized in liquid buffer or directly on membrane filters. There was no significant difference between fluorescent oligonucleotide direct counts and acridine orange direct counts (P > 0.05). Liquid buffer hybridization was preferable to direct filter hybridization.

引用

页码：3474 / 3476

页数：3

共 21 条

[1] RAPID DETECTION AND IDENTIFICATION OF VIBRIO-ANGUILLARUM BY USING SPECIFIC OLIGONUCLEOTIDE PROBE COMPLEMENTARY TO 16S RIBOSOMAL-RNA
MARTINEZ-PICADO, J
BLANCH, AR
JOFRE, J
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (02) : 732 - 737
[2] DUAL STAINING OF NATURAL BACTERIOPLANKTON WITH 4',6-DIAMIDINO-2-PHENYLINDOLE AND FLUORESCENT OLIGONUCLEOTIDE PROBES TARGETING KINGDOM-LEVEL 16S RIBOSOMAL-RNA SEQUENCES
HICKS, RE
AMANN, RI
STAHL, DA
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) : 2158 - 2163
[3] A STAPHYLOCOCCUS-AUREUS-SPECIFIC OLIGONUCLEOTIDE PROBE DERIVED FROM 16S RIBOSOMAL-RNA GENE-SEQUENCES
BENTLEY, RW
HARLAND, NM
LEIGH, JA
COLLINS, MD
LETTERS IN APPLIED MICROBIOLOGY, 1993, 16 (04) : 203 - 206
[4] TOWARDS A PHYLOGENY OF THE GENUS VIBRIO BASED ON 16S RIBOSOMAL-RNA SEQUENCES
DORSCH, M
LANE, D
STACKEBRANDT, E
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01): : 58 - 63
[5] OLIGONUCLEOTIDE PROBES FOR BORDETELLA-BRONCHISEPTICA BASED ON 16S RIBOSOMAL-RNA SEQUENCES
TANEDA, A
FUTO, S
MITSUSE, S
SETO, Y
OKADA, M
SAKANO, T
VETERINARY MICROBIOLOGY, 1994, 42 (04) : 297 - 305
[6] A SPECIFIC OLIGONUCLEOTIDE PROBE BASED ON 5S RIBOSOMAL-RNA SEQUENCES FOR IDENTIFICATION OF VIBRIO-ANGUILLARUM AND VIBRIO-ORDALII
ITO, H
ITO, H
UCHIDA, I
SEKIZAKI, T
TERAKADO, N
VETERINARY MICROBIOLOGY, 1995, 43 (2-3) : 167 - 171
[7] THE INFLUENCE OF OLIGONUCLEOTIDE-EFFECTOR ON THE SELECTIVITY OF SEQUENCE SPECIFIC MODIFICATION OF 16S RIBOSOMAL-RNA
ZENKOVA, MA
FEDOROVA, OS
LEVINA, AS
MAMAEV, SV
KARPOVA, GG
FEBS LETTERS, 1990, 269 (01) : 26 - 28
[8] RAPID IDENTIFICATION OF STAPHYLOCOCCUS-SAPROPHYTICUS USING AN OLIGONUCLEOTIDE PROBE COMPLEMENTARY TO 16S RIBOSOMAL-RNA
GASZEWSKAMASTALARZ, A
ZAKRZEWSKACZERWINSKA, J
MORDARSKI, M
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1995, 18 (01) : 123 - 126
[9] RAPID IDENTIFICATION OF RENIBACTERIUM-SALMONINARUM USING AN OLIGONUCLEOTIDE PROBE COMPLEMENTARY TO 16S RIBOSOMAL-RNA
MATTSSON, JG
GERSDORF, H
JANSSON, E
HONGSLO, T
GOBEL, UB
JOHANSSON, KE
MOLECULAR AND CELLULAR PROBES, 1993, 7 (01) : 25 - 33
[10] A 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBE FOR THE DIFFERENTIATION OF STAPHYLOCOCCUS-HAEMOLYTICUS FROM S WARNERI AND S HOMINIS
DRUEL, B
WEIZENEGGER, M
LUDWIG, W
FRENEY, J
SCHLEIFER, KH
FLEURETTE, J
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1992, 15 (04) : 535 - 537

← 1 2 3 →