ALPHA-AMYLASE AS A MARKER FOR EVALUATING THE STABILITY OF RECOMBINANT YEAST IN LONG-TERM CULTIVATION

被引：4
|
作者：
CHEN, DC
YANG, BC
CHOW, CW
KUO, TT
机构：
[1] ACAD SINICA, INST BOT, TAIPEI 11529, TAIWAN
[2] NATL TAIWAN UNIV, GRAD INST BOT, TAIPEI, TAIWAN
[3] ACAD SINICA, INST MOL BIOL, TAIPEI 11529, TAIWAN
来源：
JOURNAL OF BIOTECHNOLOGY | 1993年 / 29卷 / 03期
关键词：
RECOMBINANT YEAST; ALPHA-AMYLASE; STABILITY;
D O I：
10.1016/0168-1656(93)90064-T
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary alpha-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-forming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2mu directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the alpha-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.
引用
收藏
页码：329 / 334
页数：6
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