CONTRIBUTION OF INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) TO THE PATHOGENESIS OF SPLANCHNIC ARTERY-OCCLUSION SHOCK IN THE RAT

1 It has been suggested that leukocytes play a key role in the pathogenesis of splanchnic artery occlusion shock. Intercellular adhesion molecule 1 (ICAM-1) is an adhesion molecule of crucial importance in the phenomenon of leukocyte accumulation. 2 We investigated the involvement of ICAM-1 in the pathogenesis of splanchnic artery occlusion shock. Splanchnic artery occlusion (SAG) shock was induced in anaesthetized rats by clamping splanchnic arteries for 45 min. Sham-operated animals were used as controls. Survival time, serum tumour necrosis factor-alpha (TNF-alpha), white blood cell (WBC) count, mean arterial blood pressure, myeloperoxidase activity (MPO; studied as a quantitative means to assess leukocyte accumulation) and the responsiveness to acetylcholine of aortic rings were investigated. SAO shocked rats had a decreased survival time (90 +/- 9.5 min, while sham-shocked rats survived more than 4 h), reduced mean arterial blood pressure, increased serum levels of TNF-alpha (201 +/- 10 u ml(-1)) and MPG activity in the ileum (0.15 +/- 0.03 u x 10(-3) per g tissue) and in the lung (1.9 +/- 0.8 u x 10(-3) per g tissue), leukopenia and reduced responsiveness to acetylcholine (ACh, 10 nm-10 mu M) of aortic rings. 3 Administration of monoclonal antibody raised against rat ICAM-1 significantly increased survival time (225 +/- 9 min), reduced leukopenia and MPO activity both in the ileum (0.031 +/- 0.003 u x 10(-3) per g tissue) and in the lung 0.23 +/- 0.03 u x 10(-3) per g tissue), improved the cardiovascular changes and restored the responsiveness to ACh of aortic rings. 4 Our findings are consistent with an involvement of adhesion mechanisms in vivo in the pathogenesis of SAO shock and suggest that specific adhesion mechanisms, which support leukocyte accumulation, may represent potentially important therapeutic targets in circulatory shock.