DIFFERENTIAL-EFFECTS OF CYSTEAMINE ON HEAT-SHOCK PROTEIN INDUCTION AND CYTOPLASMIC GRANULATION IN ASTROCYTES AND GLIOMA-CELLS

被引：36
|
作者：
CHOPRA, VS
CHALIFOUR, LE
SCHIPPER, HM
机构：
[1] SIR MORTIMER B DAVIS JEWISH HOSP,LADY DAVIS INST MED RES,BLOOMFIELD CTR RES AGING,MONTREAL,PQ H3T 1E2,CANADA
[2] MCGILL UNIV,DEPT NEUROL & NEUROSURG,MONTREAL,PQ,CANADA
[3] MCGILL UNIV,DEPT MICROBIOL & IMMUNOL,MONTREAL,PQ,CANADA
[4] MCGILL UNIV,MCGILL CTR STUDIES AGING,MONTREAL,PQ,CANADA
来源：
MOLECULAR BRAIN RESEARCH | 1995年 / 31卷 / 1-2期
基金：
英国医学研究理事会;
关键词：
ASTROCYTE; CYSTEAMINE; GLIOMA; HEAT SHOCK PROTEIN; HEME OXYGENASE; INCLUSION;
D O I：
10.1016/0169-328X(95)00049-X
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The sulfhydryl agent, cysteamine (CSH), promotes the accumulation of autofluorescent, peroxidase-positive cytoplasmic granules in cultured astroglia akin to those which naturally accumulate in astrocytes of the aging periventricular brain. Both in vitro and in situ, CSH rapidly induces various heat shock proteins (HSP) in astrocytes long before granulation occurs. In the present study, we determined that CSH treatment resulted in an increase in HSP 27, HSP 90 and heme oxygenase (HO-1) at both the protein and mRNA level. We also showed that C6 glioma cells, unlike primary astrocytes, constitutively express HSP 27, HSP 90 and HO-1 at low levels. Moreover, CSH is incapable of eliciting further HSP expression or inducing granulation in the glioma cells. Our results support the hypothesis that the biogenesis of redox-active astrocytic inclusions in CSH-treated glial cultures and in the aging periventricular brain is dependent on an antecedent cellular stress response.
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页码：173 / 184
页数：12
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