TOLUENE DIISOCYANATE-INDUCED CONFORMATIONAL-CHANGES OF SERUM-ALBUMIN - A STUDY ON REPEATED INHALATIONS IN GUINEA-PIGS

被引：10
|
作者：
KOCHMAN, S
LEFEBVRE, S
BERNARD, J
MAUJEAN, A
CAZABAT, A
LAVAUD, F
MANFAIT, M
机构：
[1] CHU REIMS, FAC PHARM, SPECT BIOMOLEC LAB, F-51100 REIMS, FRANCE
[2] CHU REIMS, FAC SCI EXACTES & NAT, OENOL LAB, F-51100 REIMS, FRANCE
[3] CHU REIMS, INST JEAN GODINOT, IMMUNOL LAB, F-51100 REIMS, FRANCE
来源：
TOXICOLOGY LETTERS | 1990年 / 50卷 / 2-3期
关键词：
Fourier transform infrared spectroscopy; Guinea-pig; Serum albumin; Toluene diisocyanate;
D O I：
10.1016/0378-4274(90)90007-9
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
High responder lines of Hartley guinea-pigs were sensitized by repeated inhalations of toluene diisocyanate (TDI). After 3 weeks, we demonstrated a degree of TDI substitution of the serum-albumin-enriched fraction (AEP) and we ascertained the sensitization of the most exposed animals using PCA methodology. Fourier transform infrared spectroscopy (FT-IR), used to investigate conformational changes in AEF, highlighted the structural modifications of the native protein conformation. Such crucial changes may support, at least in part, the relationship between TDI exposure and triggering of hypersensitivity reactions. © 1990.
引用
收藏
页码：165 / 171
页数：7
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