A NEW ASSAY SYSTEM FOR THE CELL-GROWTH PROMOTING EFFECTS OF INSULIN AND GROWTH-FACTORS

被引：0

作者：

ZHANG, X ^{[1
]}

XIA, SL ^{[1
]}

TOMOOKA, Y ^{[1
]}

SAKAKURA, T ^{[1
]}

JING, NH ^{[1
]}

机构：

[1] ACAD SINICA, SHANGHAI INST BIOCHEM, SHANGHAI 200031, PEOPLES R CHINA

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 1995年 / 27卷 / 05期

关键词：

INSULIN; EPIDERMAL GROWTH FACTOR; INSULIN-LIKE GROWTH FACTOR I; BIOASSAY SYSTEM; SERUM-FREE MEDIUM;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GR2H6 is a fibroblast-like cell line cloned from a GR mouse strain mammary tumor, which can grow in a chemically defined serum-free medium. In this serum-free medium, we have found that GR2H6 cells require insulin or insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) and respond Oo these factors in a dose-related manner as measured by the increase of cell number and total DNA. Based on these observations, me have see up a bioassay system for the growth promoting activities of insulin, EGF and IGF-I. Compared with other bioassay systems this system has several advantages, such as higher sensitivity, better reproducibility and feasibility for different factors. It may provide a novel model system to study growth promoting activities of insulin and other growth factors.

引用

页码：487 / 492

页数：6

共 14 条

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