THE SENSITIVE DETERMINATION OF ABANOQUIL IN BLOOD BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY

被引：44
|
作者：
KAYE, B
CLARK, MWH
CUSSANS, NJ
MACRAE, PV
STOPHER, DA
机构：
[1] Department of Drug Metabolism Pfizer Central Research, Sandwich, Kent
来源：
BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 11期
关键词：
D O I：
10.1002/bms.1200211110
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A method is described for the determination of abanoquil in human blood. The method is based on high-performance liquid chromatography(HPLC)/atmospheric pressure positive ion chemical ionization mass spectrometry, using (H-2(3))abanoquil as internal standard. Multiple reaction monitoring is employed for selectivity and sensitivity, which enables quantification over the range 10-500 pg ml-1 with acceptable precision and accuracy. This assay methodology illustrates the versatility of atmospheric pressure ionization/tandem mass spectrometry, in conjunction with HPLC, for the separation and quantification of drugs in the subnanogram per millilitre range.
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页码：585 / 589
页数：5
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