MULTIPLE ENDO-BETA-1,4-GLUCANASE-ENCODING GENES FROM BACILLUS-LAUTUS PL236 AND CHARACTERIZATION OF THE CELB GENE

被引：16
|
作者：
JORGENSEN, PL [1 ]
HANSEN, CK [1 ]
机构：
[1] TECH UNIV DENMARK, DEPT MICROBIOL, DK-2800 LYNGBY, DENMARK
来源：
GENE | 1990年 / 93卷 / 01期
关键词：
cellulose degradation; endocellulase; enzyme; gene cloning; nucleotide sequence; Recombinant DNA;
D O I：
10.1016/0378-1119(90)90135-E
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A Bacillus lautus strain was isolated from compost by its ability to degrade microcrystalline Avicel cellulose and acid-swollen cellulose. Three DNA fragments cloned in Escherichia coli encoded at least four endo-β-1,4-glucanases (EG), of which at least two were contained on one DNA fragment. Another fragment, of 2.5 kb and carrying celB, was cloned in the shuttle-vector plasmid, pJKK3-1, and expressed in E. coli and Bacillus subtilis. The fragment was sequenced and shown to encode a 62-kDa protein, which was found as a 56-kDa mature and active EG in extracts of E. coli and in the supernatant of B. subtilis. The deduced amino acid (aa) sequence has a homology of 37% identical aa on a stretch of 295 aa to EG-E of Clostridium thermocellum. A low level of homology is detected with the Bacillus-type EG. © 1990.
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页码：55 / 60
页数：6
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