DAMAGE TO RED-BLOOD-CELLS BY HALOCOMPOUNDS

Twenty-five halocompounds were screened as inducers of hemoglobin degradation and lipid peroxidation in rat red blood cells (RBC). RBC activated halocompounds, and oxyhemoglobin remaining depended on the halocompounds employed. Thiobarbituric-acid-reactive substances (TBARS), an index of lipid peroxidation, also depended on the halocompound. TBARS were inversely correlated and oxyhemoglobin percentage was directly correlated with the logarithm of oral LD50 of the halocompounds (r = 0.79, P < 0.001, and r = 0.87, P < 0.001, respectively). For 15 halocompounds, the oral LD50 calculated as log LD50 = 0.015 (% hemoglobin) - 0.05 (TBARS) were correlated (r = 0.88, P < 0.01) with the reported oral LD50. © 1990.