IDENTIFICATION AND MAPPING OF 6 MICRODISSECTED GENOMIC DNA PROBES TO THE PROXIMAL REGION OF MOUSE CHROMOSOME-1

Six independent DNA probes, λMm1C-150, λMm1C-153, λMm1C-156, λMm1C-162, λMm1C-163, and λMm1C-165, have been isolated from a library of microdissected fragments from mouse chromosome 1, spanning cytogenetic bands C2 to C5. These DNA probes have been mapped by restriction fragment length polymorphism analysis with respect to 12 marker loci previously assigned to this portion of mouse chromosome 1, in a panel of 251 segregating Mus spretus × C57BL/6J interspecific backcross mice. The gene order and intergene distances were determined by segregation analysis to be centromere-λMm1C-162-11.1 cM-Col3a1-8.8 cM-Len-2-2.6 cM-λMm1C-163-1.6 cM-Fn-1-1.6 cM-Tp-1-0.8 CM-λMm1C-165/Vil-0.4 cM-Inha-2.8 cM-λMm1C-153-2.4 cM-λMm1C-156-1.2 cM-Pax-3-5.6 cM-Akp-3-0.8 cM-Acrg-2.0 cM-Sag-0.5 cM-Col6a3-1.8 cM-λMm1C-150-15.4 cM-Ren1,2. Four of these probes map within a chromosome 1 segment that is homologous to human chromosome 2q. Southern blotting analyses indicate that one of these anonymous probes, λMm1C-165, detects DNA fragments highly conserved across species. These novel polymorphic probes should prove useful for linkage and physical mapping of this chromosomal region. © 1992 Academic Press, Inc. All rights reserved.