Thiothymine in the synthesis of triazolotriazine and hydrazone compounds: evaluation of antimicrobial activity of some hydrazones

Background and objectives Fused triazine derivatives are known to be a very important class of compounds that have significant biological activities. Also, they showed a broad spectrum against leukemic cell lines and cancer cells. Our objective after such a study is, on one hand, to shed some light on the synthesis of triazolotriazine and hydrazone derivatives from the thiothymine compound and, on the other hand, to explore the biological activity of the isolated products from such reactions. Materials and methods Reaction of thiothymine 3 and hydrazonoyl chloride 1 in refluxing chloroform in the presence of triethylamine yielded triazolotriazine derivatives 11. The hydrazino derivative 14 underwent condensation reactions with aldehydes, pyruvic acid, ethyl pyruvate, and ketones to give the corresponding hydrazone derivatives 15 and 16, respectively. Antimicrobial activities of some newly synthesized compounds were studied using the diffusion plate method. Results and conclusion Both antibacterial and antifungal activities of the new synthesized compounds 14, 15h, and 16a - d were studied. Also, the minimum inhibitory concentration values for the highly efficient antibacterial compounds using the most sensitive microorganisms were determined.