LIQUID-CHROMATOGRAPHIC ATMOSPHERIC-PRESSURE CHEMICAL IONIZATION MASS-SPECTROMETRIC ANALYSIS OF SYNTHETIC ELASTASE INHIBITOR PEPTIDE

被引：4
|
作者：
KASUYA, F
IGARASHI, K
FUKUI, M
FUKUMORI, Y
TOKUMITO, H
FUKUDA, T
TSUDA, Y
OKADA, Y
机构：
[1] Faculty of Pharmaceutical Science, Kobe-gakuin University, Arise, Kobe, 651-21, Ikawadani, Nishi-ku
来源：
BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 10期
关键词：
D O I：
10.1002/bms.1200211006
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A liquid chromatographic/atmospheric pressure chemical ionization mass spectrometric method for the determination of tert-butyloxycarbonyl-Tyr-Leu-Val-CH2Cl (Boc-YLV-CH2Cl) was investigated. Boc-YLV-CH2Cl was eluted from a Cosmosil 5C-8 column in a solvent system of 0.1% acetic acid-methanol (30:70, v/v). The positive ion spectrum of Boc-YLV-CH2Cl showed a quasi-molecular ion at m/z 526 with fragment ions which provided structural information. Boc-YLV-CH2Cl was purified from blood and tissue samples with Sep-Pak C18 cartridges. The analysis in biological samples was performed by focusing the characteristic ion at m/z 526. The calibration graphs were linear over the concentration range studied (injected amounts of 9.5-190 pmol). The method was applied to the determination of Boc-YLV-CH2Cl in biological samples.
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页码：500 / 504
页数：5
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