DNA-REPLICATION IN ESCHERICHIA-COLI GYRB(TS) MUTANTS ANALYZED BY FLOW-CYTOMETRY

被引：10
|
作者：
VONFREIESLEBEN, U
RASMUSSEN, KV
机构：
[1] University If Institute f Microbiology, DK-1353 Copenhagen K
来源：
RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 2-3期
关键词：
DNA; REPLICATION; INITIATION; GYRB(TS) MUTANT; DECATENATION; FLOW CYTOMETRY;
D O I：
10.1016/0923-2508(91)90034-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have investigated the initiation of DNA replication in Escherichia coli gyrB (Ts) mutants and find that initiations in single cells, even those that occur at non-permissive temperature, are synchronous. Furthermore, our results indicate that the gradual arrest of DNA replication at non-permissive temperature reflects a general decrease in transcription activity and not an initiation-specific function of the DNA gyrase. At an intermediate temperature, the only effect observed was a lack of segregation (decatenation) of replicated chromosomes in a sizeable fraction of the cell population.
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页码：223 / 227
页数：5
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