SEPARATION AND ANALYSIS OF PROTEINS BY PERFUSION LIQUID-CHROMATOGRAPHY AND ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：9
|
作者：
BANKS, JF
机构：
[1] Analytica of Branford, Inc., Branford, CT 06405
来源：
JOURNAL OF CHROMATOGRAPHY A | 1995年 / 691卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)01033-B
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Perfusion LC has been interfaced with electrospray ionization mass spectrometry (ESI-MS) for the rapid separation and molecular mass determination of proteins. The combination of this unique chromatographic system and the multiply charged envelope of the ESI-MS data allows this analysis to be accomplished in just a few minutes when using the optimum conditions determined. The performance of the perfusion system has been compared to both 1 mm I.D. and 320 mu m I.D. columns packed with conventional C-18 media.
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页码：325 / 330
页数：6
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