STRUCTURAL CHARACTERIZATION AND OXYGEN BINDING-PROPERTIES OF EXTRACELLULAR HEMOGLOBIN FROM THE MARINE POLYCHAETE EURYTHOE-COMPLANATA

被引：5
|
作者：
ILAN, E [1 ]
AZEM, A [1 ]
DANIEL, E [1 ]
机构：
[1] TEL AVIV UNIV, GEORGE S WISE FAC LIFE SCI, DEPT BIOCHEM, IL-69978 TEL AVIV, ISRAEL
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 96卷 / 04期
关键词：
D O I：
10.1016/0305-0491(90)90231-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. 1. Extracellular hemoglobin from the polychaete Eurythoe complanata has a sedimentation coefficient of 54 S. In the electron microscope, the molecule exhibits a two-layered hexagonal appearance characteristic of annelid extracellular hemoglobins. 2. 2. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol gave two main bands corresponding to mol. wts 21,000 and 34,000 and a faint band corresponding to mol. wt 25,000. 3. 3. In the pH range 4.6-10.5, oxygen binding showed little co-operativity (Hill coefficient ≤1.7). Small alkaline (φ = -0.09) and reverse acid (φ = 0.04) Bohr effects were observed. 4. 4. The oxygen equilibrium properties of Eurythoe hemoglobin were compared with those of other polychaete hemoglobins. © 1990.
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收藏
页码：783 / 786
页数：4
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