MOLECULAR-CLONING OF CDNA-ENCODING HUMAN DNA HELICASE Q1 WHICH HAS HOMOLOGY TO ESCHERICHIA-COLI REC-Q HELICASE AND LOCALIZATION OF THE GENE AT CHROMOSOME-12P12

被引：142
|
作者：
SEKI, M
MIYAZAWA, H
TADA, S
YANAGISAWA, J
YAMAOKA, T
HOSHINO, S
OZAWA, K
EKI, T
NOGAMI, M
OKUMURA, K
TAGUCHI, H
HANAOKA, F
ENOMOTO, T
机构：
[1] UNIV TOKYO, FAC PHARMACEUT SCI, DEPT PHYSIOL CHEM, BUNKYO KU, TOKYO 113, JAPAN
[2] INST PHYS & CHEM RES, CELLULAR PHYSIOL LAB, WAKO, SAITAMA 35101, JAPAN
[3] INST PHYS & CHEM RES, DIV HUMAN GENOME RES, TSUKUBA, IBARAKI 305, JAPAN
[4] INST PHYS & CHEM RES, GENE BANK, TSUKUBA, IBARAKI 305, JAPAN
[5] MIE UNIV, FAC BIORESOURCES, BIOL CHEM LAB, TSU, MIE 514, JAPAN
来源：
NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 22期
关键词：
D O I：
10.1093/nar/22.22.4566
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A complementary DNA encoding DNA-dependent ATPase Q1 possessing DNA helicase activity, which is the major DNA-dependent ATPase in human cell extracts, was cloned from a cDNA library of human KB cells. The predicted amino acid sequence has seven consecutive motifs conserved in the RNA and DNA helicase super family and DNA helicase Q1 belongs to DEXH helicase family. A homology search indicated that helicase Q1 had 47% homology in the seven conserved regions with Escherichia coli RecQ protein. Three RNA bands of 4.0, 3.3, and 2.2 kilobases were detected in HeLa cells by Northern blotting. Analysis of the genomic DNA indicated the presence of a homologous gene in mouse cells. The DNA helicase Q1 gene was localized on the short arm of human chromosome 12 at 12p12.
引用
收藏
页码：4566 / 4573
页数：8
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