COMPARATIVE SEQUENCE OF THE HELPER COMPONENT (HC) REGION OF POTATO VIRUS-Y AND A HC-DEFECTIVE STRAIN, POTATO VIRUS-C

被引：77
|
作者：
THORNBURY, DW
PATTERSON, CA
DESSENS, JT
PIRONE, TP
机构：
[1] Department of Plant Pathology, University of Kentucky, Lexington
来源：
VIROLOGY | 1990年 / 178卷 / 02期
关键词：
D O I：
10.1016/0042-6822(90)90356-V
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Potato virus C (PVC), a non-aphid transmissible strain of potato virus Y (PVY), was found to code for a protein (PVC-HC) which is similar in molecular weight and immunological reactivity to the helper component protein of PVY (PVY-HC). PVC-HC, however, was inactive with respect to its ability to effect aphid transmission of either PVC or PVY. The 5′-terminal 2.7-kb regions of PVC and PVY were sequenced. Within the HC region there was 92% nucleotide homology between the two strains; comparison of the derived amino acid sequences revealed 24 amino acid differences. Comparison of the PVC-HC sequence with that of five potyviruses revealed 2 amino acid changes which were specific to PVC-HC. These amino acids are prime targets for mutational analysis of HC activity. © 1990.
引用
收藏
页码：573 / 578
页数：6
相关论文
共 50 条
12345