Optimization of Enzymatic Clarification from Corncob

被引：0
|
作者：
Chongkhong, Sininart [1 ]
Kongjindamunee, Woraluk [1 ]
机构：
[1] Prince Songkla Univ, Dept Chem Engn, Fac Engn, Hat Yai, Thailand
来源：
INTERNATIONAL TRANSACTION JOURNAL OF ENGINEERING MANAGEMENT & APPLIED SCIENCES & TECHNOLOGIES | 2014年 / 5卷 / 01期
关键词：
Alpha-amylase; Glucose content; Hydrolysis; Central composite design;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A major content that was 22.76% total carbohydrate of the corncob could be simply hydrolyzed into reducing sugars by using alpha-amylase. The clarification process using alpha-amylase was optimized by response surface methodology (RSM) in this work. Independent variables including: enzyme amount of 0.05-0.2 %w, time of 60-240 min and temperatures of 80-100 degrees C were investigated. Their effects were found on the reducing sugar (Glucose content) by a second order central composite design (CCD). The optimum condition was 0.2 %w alpha-amylase, 87.6 degrees C for 150 min. It could provide the highest amount of 6.21 g/L glucose content in the clarified product. (C) 2014 INT TRANS J ENG MANAG SCI TECH.
引用
收藏
页码：67 / 76
页数：10
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