ROUTINE MICROBIOLOGICAL ASSAY FOR CARNITINE ACTIVITY IN BIOLOGICAL-FLUIDS AND TISSUES

被引：7
|
作者：
BAKER, H [1 ]
DEANGELIS, B [1 ]
BAKER, ER [1 ]
REDDI, AS [1 ]
KHALIL, M [1 ]
FRANK, O [1 ]
机构：
[1] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT MED,NEWARK,NJ 07103
来源：
FOOD CHEMISTRY | 1992年 / 43卷 / 02期
关键词：
D O I：
10.1016/0308-8146(92)90228-T
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A practical method for measuring acid-soluble and total (acid- and alkaline-soluble) carnitine growth activity for a carnitine-specific mutant of the enteric yeast Torulopsis bovina in biological fluids and tissues is described. It grows at 37-degrees-C in a simple, cheap, chemically defined medium; its response threshold to carnitine is 100 pg ml-1. Growth is measured as absorbance units with any turbidometer. Carnitine contents in human and rat fluids and tissues so measured accord with those obtained after multi-step extraction methods, enzyme procedures or radiochemical procedures.
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页码：141 / 146
页数：6
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