Applicability of alkaline protease and alkaline amylase of Bacillus agaradhaerens MTCC 9416

The advances in the application of alkalophilic enzymes are due to their ability to showactivity in the alkaline range of pH. This property has made themuseful in detergent industry as detergent enzymes usually have a pH in solution of between 8.0 and 10.5. The destaining efficiency of too alkaline enzymes, viz, alkaline protease and alkaline amylase, produced by an alkalaphilic bacterium Bacillus agaradhaerens MCC 9416 was determined. It was found that among the different conditions of washing tested; the mixture of alkaline protease and alkaline amylase produced by Bacillus agaradhaerens showed faintness of the blood, chocolate spots, pasta residues and sauce spots on the cloth. Furthermore, the set containing the combination of detergent and enzymes of Bacillus agaradhaerens also exhibited good results. The results of de-staining experiment showed the complete removal of stain in detergent solution supplemented oath enzyme whereas the stains were not completely removed fromcloth dipped in detergent solution only. This suggested that the enzymes nay be useful to detergent industry