THE CYTOKININ CONTENT IN NEEDLES OF NORWAY SPRUCE (PICEA-ABIES [L] KARST) WITH DIFFERENT DEGREES OF DAMAGE

被引:9
|
作者
VONSCHWARTZENBERG, K [1 ]
HAHN, H [1 ]
机构
[1] UNIV HAMBURG,INST ANGEW BOT,W-2000 HAMBURG 36,GERMANY
关键词
PICEA-ABIES (L) KARST; SPRUCE; CYTOKININS; FOREST DECLINE; ENZYME-LINKED IMMUNOSORBENT ASSAY;
D O I
10.1016/S0176-1617(11)80611-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In order to check for relations between tree damage and changes in plant hormone concentrations the cytokinins t-zeatin, t-zeatin riboside, isopentenyladenine and isopentenyladenosine were examined. Needles of young and old spruce from forest plantations showing different degrees of novel forest decline were analysed using chromatographic prepurification, HPLC-separation and quantification by means of an indirect competitive enzyme-linked immunosorbent assay. One year time course analyses revealed strongly elevated concentrations of t-zeatin riboside and isopentenyladenosine in diseased trees throughout the whole year. Annual mean values for the cytokinin ribosides in 1-year-old needles of old spruce were: heavily damaged - t-zeatin riboside 371, isopentenyladenosine 914; slightly damaged - 42, 202 (respectively, in pmol/g dry weight). The developed ELISA may be used as a diagnostic tool to detect changes in the health of trees not detectable by conventional methods.
引用
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页码:218 / 223
页数:6
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