IN-SITU PREPARATION OF FATTY-ACID METHYL-ESTERS FOR ANALYSIS OF FATTY-ACID COMPOSITION IN FOODS

被引：392
|
作者：
PARK, PW
GOINS, RE
机构：
[1] Mead Johnson Research Center, Mead Johnson Nutritional Group, Evansville, Indiana, 47721
来源：
JOURNAL OF FOOD SCIENCE | 1994年 / 59卷 / 06期
关键词：
FATTY ACID; METHYL ESTERS; IN SITU TRANSESTERIFICATION; GAS CHROMATOGRAPHY;
D O I：
10.1111/j.1365-2621.1994.tb14691.x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Lipid extraction preceding fatty acid methyl esters (FAME) preparation for gas chromatography is time-consuming and cumbersome. We omitted the lipid extraction and performed in situ transesterification (ISTE) by heating lipid-containing foods at 90 degrees C for 10 min after adding 0.5N NaOH in methanol for methanolysis and continued heating another 10 min for further methylation after adding 14% BF3 in methanol. FAME prepared by ISTE showed fatty acid composition virtually identical to FAME prepared after lipid extraction from powder, liquid, phospholipid-rich, and tissue products. Due to its simplicity, speed, and reduced organic solvent usage, ISTE should be useful to determine overall fatty acid composition of foods.
引用
收藏
页码：1262 / 1266
页数：5
相关论文
共 50 条
  • [1] METHYL-ESTERS IN THE FATTY-ACID INDUSTRY
    FARRIS, RD
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1979, 56 : A770 - A773
  • [2] ROBOTIC WORKSTATION FOR THE PREPARATION OF FATTY-ACID METHYL-ESTERS
    DEPALMA, RA
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1987, 25 (05) : 219 - 222
  • [3] ALCOHOLYSIS, SAPONIFICATION AND PREPARATION OF FATTY-ACID METHYL-ESTERS
    GLASS, RL
    [J]. LIPIDS, 1971, 6 (12) : 919 - &
  • [4] FATTY-ACID METHYL-ESTERS IN LEAF LIPIDS
    MALLET, JF
    UCCIANI, E
    GAMISANS, J
    [J]. REVUE FRANCAISE DES CORPS GRAS, 1993, 40 (3-4): : 121 - 123
  • [5] THE ANTICLASTOGENIC POTENTIAL OF FATTY-ACID METHYL-ESTERS
    RENNER, HW
    [J]. MUTATION RESEARCH, 1986, 172 (03): : 265 - 269
  • [6] FATTY-ACID METHYL-ESTERS IN PHOTOSYNTHETIC BACTERIA
    MAUDINAS, B
    VILLOUTREIX, J
    [J]. PHYTOCHEMISTRY, 1977, 16 (08) : 1299 - 1300
  • [7] CHARACTERIZATION OF FATTY-ACID METHYL-ESTERS BY THERMAL-ANALYSIS
    REY, FJ
    CHAMORRO, O
    GIL, FJM
    GIL, JM
    [J]. JOURNAL OF THERMAL ANALYSIS, 1993, 40 (02): : 463 - 473
  • [8] GAS-CHROMATOGRAPHIC ANALYSIS OF FATTY-ACID METHYL-ESTERS
    EDER, K
    [J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 671 (1-2): : 113 - 131
  • [9] DIFFUSION OF METHYL-ESTERS OF HIGHER FATTY-ACID IN POLYPROPYLENE
    HAYASHI, H
    SAKAI, H
    MATSUZAWA, S
    [J]. JOURNAL OF APPLIED POLYMER SCIENCE, 1994, 51 (13) : 2165 - 2173
  • [10] CARCINOGENICITY IN MICE OF SOME FATTY-ACID METHYL-ESTERS
    ARFFMANN, E
    [J]. ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA SECTION A-PATHOLOGY, 1974, A 82 (01): : 127 - 136
12345