DIRECTION OF DNA ENTRY IN COMPETENT CELLS OF BACILLUS-SUBTILIS

被引：12
|
作者：
VAGNER, V [1 ]
CLAVERYS, JP [1 ]
EHRLICH, SD [1 ]
MEJEAN, V [1 ]
机构：
[1] UNIV TOULOUSE 3,CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,F-31062 TOULOUSE,FRANCE
来源：
MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 10期
关键词：
D O I：
10.1111/j.1365-2958.1990.tb00557.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Direction of DNA entry in Bacillus subtilis competent cells was studied using molecules in which only one of the two strands was radioactively labelled. The label was either distributed homogeneously or was localized in a small region of the strand, in the centre or at one of the ends. Regardless of the distribution and the position of the label, similar amounts of radioactivity were taken up by the cells exposed to the labelled molecules. This suggests that DNA enters B. subtilis either by two different uptake systems having opposite polarities, or by a single non‐polar system. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：1785 / 1788
页数：4
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