NYLON-LINAMARASE ELECTRODE FOR RAPID-DETERMINATION OF LINAMARIN

被引：16
|
作者：
YEOH, HH
机构：
[1] Department of Botany, National University of Singapore, Kent Ridge, Singapore
来源：
BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 10期
关键词：
D O I：
10.1007/BF00152627
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme electrode was constructed using cassava leaf linamarase covalently linked via polyethyleneimine to Hybond-N nylon. The nylon-enzyme electrode response was Nerstian for linamarin range of 0.1 to 20 mM. A steady state reading could be obtained within 4 to 6 mins. The nylon-enzyme discs could be reused. Compared to the previously reported enzyme electrode prepared by entrappment of linamarase in ENT-4000 prepolymer resins, the nylon-enzyme electrode gave faster response and could save analysis time by 60%.
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页码：761 / 764
页数：4
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