A GENOMIC CLONE OF A TYPE-I CAB GENE ENCODING A LIGHT-HARVESTING CHLOROPHYLL A/B BINDING-PROTEIN OF PHOTOSYSTEM-II IDENTIFIED FROM LODGEPOLE PINE

被引:9
|
作者
BARRETT, JW
BEECH, RN
DANCIK, BP
STROBECK, C
机构
[1] UNIV ALBERTA,DEPT FOREST SCI,EDMONTON T6H 2G1,AB,CANADA
[2] UNIV ALBERTA,DEPT ZOOL,EDMONTON T6H 2G1,AB,CANADA
关键词
CHLOROPHYLL A/B BINDING PROTEIN; GYMNOSPERM; LODGEPOLE PINE; PHOTOSYSTEM II TYPE I CAB GENE;
D O I
10.1139/g94-021
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A type I cab gene encoding chlorophyll a and b binding protein from lodgepole pine (Pinus contorta Dougl., var. latifolia (Engelm.)) has been isolated and sequenced. The gene encodes a proposed 40 amino acid transit peptide and a 234 amino acid mature CAB polypeptide. There is no evidence of an intron and examination of the type specific amino acids confirm this to be a photosystem II type I cab gene. The coding region is highly conserved with a similar gene from Scots pine, the homology extending into the 5' and 3' untranslated regions. Sequence comparison of the 1-kb region upstream of the translation start codon from the lodgepole pine cab gene exhibited little similarity to identified regulatory regions in pea, tobacco, wheat, and Arabidopsis cab genes. The cab gene, which is 62% G + C rich, is surrounded by 70-75% A + T rich regions, which may confer some regulatory feature on its expression.
引用
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页码:166 / 172
页数:7
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