INVESTIGATING EFFECT OF CATECHIN FLAVONOIDS (GREEN TEA) AND BACLOFEN ON VISCERAL PAIN INDUCED BY ACETIC ACID

This research was designed to study the twin effect of baclofenand catechin on acetic derived visceral pain in rats. In this study, adult male Wistar rats weighing 200-250 g were used, Mice were stored as groups of six rats in polyethylene cages in a room with ambient conditions and the optimum temperature about 23 +/- 2 degrees C and 12 hours of light period. All tests were performed within 8 am to 3 pm and each rat was used once for the test. Sterile normal saline, acetic acid solution 1%, cathechin and baclofen (bought from sigma company) were used in the study. Visceral pain response was investigated by intra peritoneal injection of normal saline. Visceral pain response after pre-injection of cathechin in amount of 5 mg per kg body weight before baclofen in amount of 2.5 mg per kg body weight was studied. Each completely randomized experiment was performed with six replications. Mean values were compared using GLM procedure of SAS software (9.3). Duncan test was used for comparing mean values in each test, and Dunnett method was used for comparing mean values of each experiment for the control group. Results showed that, the onset time for first contraction to start from latency period in injection of 2.5 mg baclofen per kg of body weight, and 5 mg cathechin per kg of body weight was 1001/833 seconds, and it caused 0/978 full contractions of the abdominal wall. It can be said that catechin induces analgesia by baclofen dependent mechanism.