MODIFIED NUCLEOSIDE 5'-TRIPHOSPHATES CONTAINING ADDITIONAL 2',3'-FUSED CYCLE AS SUBSTRATES FOR DNA-POLYMERASES

被引：0
|
作者：
SEMIZAROV, DG
VIKTOROVA, LS
KRAEVSKII, AA
KUKHANOVA, MK
机构：
来源：
MOLECULAR BIOLOGY | 1994年 / 28卷 / 01期
关键词：
REVERSE TRANSCRIPTASE; NUCLEOSIDE 5'-TRIPHOSPHATE ANALOGS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It was shown that 5'-triphosphates of 1-(2',3'-epithio-2',3'-dideoxy-beta-D-lyxofuranosyl)thymine (ItTTP), 1-(2',3'-epithio-2',3'-dideoxy-beta-D-ribofuranosyl)thymine (rtTTP), and 2',3'-lyxoanhydrothymidine (laTTP) are terminating substrates for HIV and AMV reverse transcriptases and E. coli DNA polymerase I, but do not terminate DNA synthesis catalyzed by DNA polymerase E from human placenta. The Michaelis constant for incorporation of ltTTP, rtTTP, and laTTP into the growing DNA chain are close to each other and 1.5-2.5-fold higher than that for dTTP. Their V-max values are only two-threefold lower than that for dTTP. The data obtained support the hypothesis that modified substrates with a flattened glycon have high affinity to the active centers of DNA polymerases.
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页码：75 / 81
页数：7
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