AMPEROMETRIC DETERMINATION OF PHOSPHATE BY USE OF A NUCLEOSIDE PHOSPHORYLASE XANTHINE-OXIDASE ENZYME SENSOR BASED ON A CLARK-TYPE HYDROGEN-PEROXIDE OR OXYGEN-ELECTRODE

被引：29
|
作者：
HAEMMERLI, SD
SULEIMAN, AA
GUILBAULT, GG
机构：
[1] University of New Orleans, Department of Chemistry, New Orleans
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 191卷 / 01期
关键词：
D O I：
10.1016/0003-2697(90)90395-P
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A bienzymatie sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 μm. © 1990.
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页码：106 / 109
页数：4
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