TRANSIENT EFFECT OF EPIDERMAL GROWTH-FACTOR ON THE MOTILITY OF AN IMMORTALIZED MAMMARY EPITHELIAL-CELL LINE

被引:0
|
作者
MATTHAY, MA
THIERY, JP
LAFONT, F
STAMPFER, MF
BOYER, B
机构
[1] LAB PHYSIOPATHOL DEV, CNRS, URA 1337, 46 RUE ULM, F-75230 PARIS 05, FRANCE
[2] ECOLE NORMALE SUPER, DEV & EVOLUT SYST NERVEUX LAB, CNRS, URA 1414, F-75230 PARIS 05, FRANCE
[3] UNIV CALIF BERKELEY, DIV CELL & MOLEC BIOL, BERKELEY, CA 94720 USA
关键词
EPIDERMAL GROWTH FACTOR; MAMMARY EPITHELIAL CELLS; MOTILITY; WOUND HEALING; INTEGRINS; CELL ADHESION;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effects of growth factors on epithelial cell motility and dispersion have been examined on an immortalized human mammary epithelial cell line, the 184A1 nontumorigenic cell line. Among all the molecules tested, epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) were demonstrated to stimulate an increase in mammary epithelial cell motility and wound closure that was associated with a morphological transformation of the cells and was accompanied by modifications in cell-cell and cell-substrate adhesion systems. The EGF-induced increase in cell motility and monolayer wound closure occurred over a 24 hour period and was not dependent on an increase in cell number. The effect of EGF was abolished by inhibiting alpha2 integrins with specific antibodies, indicating that part of the mechanism for the increase in cell motility and accelerated wound closure depends on alpha2 integrin functional expression. After 72 hours of exposure to EGF, the EGF-induced alterations in cell morphology, motility and cell adhesion systems underwent a spontaneous reversion that was correlated with a 10-fold reduction in the number of EGF receptors. The ability to regulate the scattering response induced by growth factors might be an important feature distinguishing normal epithelial cells from their tumoral counterparts.
引用
收藏
页码:869 / 878
页数:10
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