ONE-STEP FABRICATION OF GLUCOSE SENSORS BASED ON ENTRAPMENT OF GLUCOSE-OXIDASE WITHIN POLY(ESTER SULFONIC-ACID) COATINGS

A simple, rapid, reproducible and effective one-step procedure for biosensor construction, based on casting mixed poly(ester-sulfonic acid)-glucose oxidase solutions onto solid electrodes is described. The cation exchanger simultaneously performs the entrapment, charge permselectivity and antifouling functions. The discrimination against potential interferences is accompanied with a fast and sensitive response to glucose. With flow injection operation, 180 samples can be processed per hour, with a detection limit of 3 x 10(-6) M. The effects of numerous experimental variables are explored. These properties of poly(ester-sulfonic acid) ionomers, coupled with their availability as aqueous dispersions, make them attractive and versatile for biosensor work.