THE INFLUENCE OF ISOFLURANE ON THE SYNAPTIC ACTIVITY OF 5-HYDROXYTRYPTAMINE

被引:18
|
作者
MARTIN, DC
ADAMS, RJ
ARONSTAM, RS
机构
[1] MED COLL GEORGIA,DEPT PHYSIOL & ENDOCRINOL,AUGUSTA,GA 30912
[2] MED COLL GEORGIA,DEPT PHARMACOL & TOXICOL,AUGUSTA,GA 30912
关键词
ANESTHETICS; BRAIN; BRAIN NEUROTRANSMITTERS; 5-HYDROXYTRYPTAMINE; ISOFLURANE; SEROTONIN;
D O I
10.1007/BF00965741
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of isoflurane on uptake of 5-hydroxytryptamine (serotonin; 5-HT) by rat brain synaptosomes and binding of 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) and ketanserin to 5-HT1A and 5-HT2 receptors were examined. Isoflurane caused a concentration-dependent decrease in synaptosomal 5-HT uptake that was kinetically defined as non-competitive; exposure to isoflurane decreased V(max) but had no effect on the apparent K(m). Removal of the drug from the reaction mixture resulted in the return of 5-HT accumulation rates to control levels. Isoflurane inhibited 8-OH-DPAT binding to hippocampal membranes by up to 27 +/-6% at 4.5 mM, but not significantly affect ketanserin binding to 5-HT2 receptors. These findings suggest that presynaptic actions are more important than postsynaptic actions in the modulation of serotonergic neutrotransmission by isoflurane.
引用
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页码:969 / 973
页数:5
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