PROTECTIVE EFFECT OF S-ADENOSYL-1-METHIONINE ON LIVER-DAMAGE INDUCED BY BILIARY OBSTRUCTION IN RATS - A HISTOLOGICAL, ULTRASTRUCTURAL AND BIOCHEMICAL APPROACH

In human and experimental CCl4-liver damage, S-adenosyl-1-methionine-synthetase and/or the intrahepatic content of Sadenosyl-1-methionine, are diminished and in human cirrhosis phospholipid methyltransferase is markedly reduced. Therefore the aim of this study was to investigate the effect of S-adenosyl-1-methionine administration on liver damage induced by 15-day bile duct ligation. Liver damage was analyzed by histological, ultrastructural and biochemical techniques. Biliary obstruction produced an increase in collagen content, dilation of the bile canaliculi and disorganization of mitochondria. These effects were not observed in the bile-duct-ligated group receiving S-adenosyl-1-methionine. Biochemical results showed that bile duct ligation increased serum bilirubins, and alkaline phosphatase and gamma-glutamyl transpeptidase activities. These effects were prevented significantly by S-adenosyl-1-methionine. On the other hand, glycogen content in the liver was depleted while lipid peroxidation was increased by biliary obstruction, S-adenosyl-1-methionine administration prevented these effects. In the bile-duct-ligated group, hepatocyte and erythrocyte plasma membrane Na+/K+ and Ca2+-ATPases were lower than in the control group (p<0.05). Administration of S-adenosyl-1-methionine preserved ATPase activities. The exogenous S-adenosyl-1-methionine supply is probably responsible for restoring transmethylation lost in liver diseases. (C) Journal of Hepatology.